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strains 690  (ATCC)


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    ATCC strains 690
    Strains 690, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strains+690/Alternaria+solani+(Ellis+et+Martin)+Sorauer%2C+anamorph/pm40889854-168-0-7
    Average 94 stars, based on 1 article reviews
    strains 690 - by Bioz Stars, 2026-08
    94/100 stars

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    strains 690  (ATCC)
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    ATCC strains 690
    Strains 690, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strains+690/Alternaria+solani+(Ellis+et+Martin)+Sorauer%2C+anamorph/pm40889854-168-0-7
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    strains 690 - by Bioz Stars, 2026-08
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    competent e coli bl21 690 strain  (New England Biolabs)
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    skno 1 cell line strain  (DSMZ)
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    DSMZ skno 1 cell line strain
    A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus <t>k-mer-rich</t> <t>SKNO-1</t> SRA datasets.
    Skno 1 Cell Line Strain, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strains+690/SKNO-1/bio_rxiv__64898__2026__01__22__701047-182-15-21
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    coding sequence of truncated e2 (te2, residues 690—865) of bvdv hubei strain  (Sangon Biotech)
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    Sangon Biotech coding sequence of truncated e2 (te2, residues 690—865) of bvdv hubei strain
    Expression and identification of CSFV E rns and <t>BVDV</t> tE2 proteins. A & C The recombinant CSFV E rns ( A ) or BVDV tE2 ( C ) protein expressed in E. Coli was analyzed by SDS-PAGE, with Coomassie blue staining. M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( A ) or pET-tE2 ( C ); Lane 3, the purified CSFV E rns ( A ) or BVDV tE2 ( C ) protein. B & D Recombinant CSFV E rns ( B ) or BVDV tE2 ( D ) protein was confirmed by western blotting using an anti-His monoclonal antibody (upper) or a specific anti-virus polyclonal antibody (lower). M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( B ) or pET-tE2 ( D )
    Coding Sequence Of Truncated E2 (Te2, Residues 690—865) Of Bvdv Hubei Strain, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strains+690/coding+sequence+of+truncated+e2++te2++residues+690+865++of+bvdv+hubei+strain/pmc09308313-58-25-31
    Average 90 stars, based on 1 article reviews
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    A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus k-mer-rich SKNO-1 SRA datasets.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus k-mer-rich SKNO-1 SRA datasets.

    Article Snippet: In addition, RNA-seq dataset ERR3003594 was analyzed, as it corresponds to the dataset characterizing the SKNO-1 cell line strain obtained from DSMZ repository that was further utilized in this study.

    Techniques: Sequencing

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet:

    Article Snippet: In addition, RNA-seq dataset ERR3003594 was analyzed, as it corresponds to the dataset characterizing the SKNO-1 cell line strain obtained from DSMZ repository that was further utilized in this study.

    Techniques:

    A) Schematic representation of chromosome 21 in the SKNO-1 cell line, showing the integration site of the anellovirus genome (dotted green line). Red represents the centromere, blue indicates the repetitive region, and the grayscale sharing indicates Giemsa bands (darker shades correspond to higher heterochromatin and greater AT-rich regions). B) Detailed schematic of anellovirus integration within chromosome 21. The pink region denotes the anellovirus genome, with arrows indicating open reading frames (ORF). A grey arrow highlights ORF2 containing an early stop codon at position codon position 2, while an asterisk (*) marks the truncation of ORF1, preventing overlap with ORF3. The violet line highlights the duplicated sequences located at both termini of the viral genome, which differ by two-point mutations (C→A and G→A), while the blue region indicates an additional nucleotide segment corresponding to a MaLR-like element. C) Comparison between wild-type chromosome 12 and the chromosome 21 with the anellovirus genome integration. The integrant interrupts the RNA45SN2/RNA28SN2 rRNA gene. Dotted green lines indicate the region that has been altered within chromosome 21 including specific coordinates for the integration site. Below the schematic of chromosome 21 with the integration, PacBio long reads are shown (represented by grey rectangles), confirming the presence of the integrant in the SKNO-1 J subline cell.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Schematic representation of chromosome 21 in the SKNO-1 cell line, showing the integration site of the anellovirus genome (dotted green line). Red represents the centromere, blue indicates the repetitive region, and the grayscale sharing indicates Giemsa bands (darker shades correspond to higher heterochromatin and greater AT-rich regions). B) Detailed schematic of anellovirus integration within chromosome 21. The pink region denotes the anellovirus genome, with arrows indicating open reading frames (ORF). A grey arrow highlights ORF2 containing an early stop codon at position codon position 2, while an asterisk (*) marks the truncation of ORF1, preventing overlap with ORF3. The violet line highlights the duplicated sequences located at both termini of the viral genome, which differ by two-point mutations (C→A and G→A), while the blue region indicates an additional nucleotide segment corresponding to a MaLR-like element. C) Comparison between wild-type chromosome 12 and the chromosome 21 with the anellovirus genome integration. The integrant interrupts the RNA45SN2/RNA28SN2 rRNA gene. Dotted green lines indicate the region that has been altered within chromosome 21 including specific coordinates for the integration site. Below the schematic of chromosome 21 with the integration, PacBio long reads are shown (represented by grey rectangles), confirming the presence of the integrant in the SKNO-1 J subline cell.

    Article Snippet: In addition, RNA-seq dataset ERR3003594 was analyzed, as it corresponds to the dataset characterizing the SKNO-1 cell line strain obtained from DSMZ repository that was further utilized in this study.

    Techniques: Comparison

    Reads from ChIP-Seq SRA datasets were trimmed with fastp and aligned to a de-novo assembly of the anellovirus genome constructed from RNA-Seq data of the SKNO-1 cell line (see Methods). Alignment depth is plotted across the 3,425 bp reference genome. Plots are labeled with the associated SRA and ChIP-Seq experimental conditions (e.g. protein targeted, buffer). Predicted open reading frame coordinate intervals of the anellovirus assembly are displayed at the bottom. The span of the entire assembly is depicted by the black bar, and ORFs are shown as yellow boxes.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: Reads from ChIP-Seq SRA datasets were trimmed with fastp and aligned to a de-novo assembly of the anellovirus genome constructed from RNA-Seq data of the SKNO-1 cell line (see Methods). Alignment depth is plotted across the 3,425 bp reference genome. Plots are labeled with the associated SRA and ChIP-Seq experimental conditions (e.g. protein targeted, buffer). Predicted open reading frame coordinate intervals of the anellovirus assembly are displayed at the bottom. The span of the entire assembly is depicted by the black bar, and ORFs are shown as yellow boxes.

    Article Snippet: In addition, RNA-seq dataset ERR3003594 was analyzed, as it corresponds to the dataset characterizing the SKNO-1 cell line strain obtained from DSMZ repository that was further utilized in this study.

    Techniques: ChIP-sequencing, Construct, RNA Sequencing, Labeling

    A) Maximum-likelihood phylogenetic tree of ORF1 nucleotide sequences from Alphatorquevirus, Betatorquevirus, and Gammatorquevirus reference genomes. Ultrafast bootstrap support values (1,000 replicates) are shown for key nodes. Anellovirus assembled from the SKNO-1 cell line is highlighted in red, and branches corresponding to the clade containing this virus together with sequences retrieved from NCBI BLAST showing >90% genomic identity are shown in pink. The Betatorquevirus clade is collapsed and represented as a green triangle, while the Gammatorquevirus clade is collapsed in blue. Branch lengths correspond to the number of substitutions per site. B) P-distance matrix comparing the SKNO-1 anellovirus, the SKNO-1–like anellovirus clade, and representative sequences of Alphatorquevirus homin27, homin28, and homin29. Bootstrap standard errors (100 replicates) are shown in italics.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Maximum-likelihood phylogenetic tree of ORF1 nucleotide sequences from Alphatorquevirus, Betatorquevirus, and Gammatorquevirus reference genomes. Ultrafast bootstrap support values (1,000 replicates) are shown for key nodes. Anellovirus assembled from the SKNO-1 cell line is highlighted in red, and branches corresponding to the clade containing this virus together with sequences retrieved from NCBI BLAST showing >90% genomic identity are shown in pink. The Betatorquevirus clade is collapsed and represented as a green triangle, while the Gammatorquevirus clade is collapsed in blue. Branch lengths correspond to the number of substitutions per site. B) P-distance matrix comparing the SKNO-1 anellovirus, the SKNO-1–like anellovirus clade, and representative sequences of Alphatorquevirus homin27, homin28, and homin29. Bootstrap standard errors (100 replicates) are shown in italics.

    Article Snippet: In addition, RNA-seq dataset ERR3003594 was analyzed, as it corresponds to the dataset characterizing the SKNO-1 cell line strain obtained from DSMZ repository that was further utilized in this study.

    Techniques: Virus

    A) Multiple alignment of amino acid sequence between Betatorquevirus LY1 ORF1 and Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The identical sites are marked as black. Betatorquevirus LY1 ORF1 is annotated with purple bar, and Alphatorquevirus sp. isolate SKNO-1 ORF1 is annotated with orange and yellow bar. B) The PDB structures of Betatorquevirus LY1 ORF1 and AlphaFold predicted structure Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The motif ARM, P2, P1, JR, and C-terminal are marked on the structure. Pentamers structures are shown on the right.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Multiple alignment of amino acid sequence between Betatorquevirus LY1 ORF1 and Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The identical sites are marked as black. Betatorquevirus LY1 ORF1 is annotated with purple bar, and Alphatorquevirus sp. isolate SKNO-1 ORF1 is annotated with orange and yellow bar. B) The PDB structures of Betatorquevirus LY1 ORF1 and AlphaFold predicted structure Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The motif ARM, P2, P1, JR, and C-terminal are marked on the structure. Pentamers structures are shown on the right.

    Article Snippet: In addition, RNA-seq dataset ERR3003594 was analyzed, as it corresponds to the dataset characterizing the SKNO-1 cell line strain obtained from DSMZ repository that was further utilized in this study.

    Techniques: Sequencing

    Expression and identification of CSFV E rns and BVDV tE2 proteins. A & C The recombinant CSFV E rns ( A ) or BVDV tE2 ( C ) protein expressed in E. Coli was analyzed by SDS-PAGE, with Coomassie blue staining. M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( A ) or pET-tE2 ( C ); Lane 3, the purified CSFV E rns ( A ) or BVDV tE2 ( C ) protein. B & D Recombinant CSFV E rns ( B ) or BVDV tE2 ( D ) protein was confirmed by western blotting using an anti-His monoclonal antibody (upper) or a specific anti-virus polyclonal antibody (lower). M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( B ) or pET-tE2 ( D )

    Journal: Virology Journal

    Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

    doi: 10.1186/s12985-022-01851-w

    Figure Lengend Snippet: Expression and identification of CSFV E rns and BVDV tE2 proteins. A & C The recombinant CSFV E rns ( A ) or BVDV tE2 ( C ) protein expressed in E. Coli was analyzed by SDS-PAGE, with Coomassie blue staining. M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( A ) or pET-tE2 ( C ); Lane 3, the purified CSFV E rns ( A ) or BVDV tE2 ( C ) protein. B & D Recombinant CSFV E rns ( B ) or BVDV tE2 ( D ) protein was confirmed by western blotting using an anti-His monoclonal antibody (upper) or a specific anti-virus polyclonal antibody (lower). M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( B ) or pET-tE2 ( D )

    Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

    Techniques: Expressing, Recombinant, SDS Page, Staining, Marker, Bacteria, Control, Plasmid Preparation, Purification, Western Blot, Virus

    Optimizations of CSFV E rns and BVDV tE2 -based ELISA procedures. A & C Optimization of the concentration of coating antigen and the dilution of swine sera for CSFV E rns ( A ) or BVDV tE2 ( C ) -based ELISA using checkerboard titration test. B & D Optimization of the dilution of the secondary antibody for CSFV E rns ( B ) or BVDV tE2 ( D ) -based ELISA. P/N, positive control/negative control; ☆, the optimized condition for ELISA test

    Journal: Virology Journal

    Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

    doi: 10.1186/s12985-022-01851-w

    Figure Lengend Snippet: Optimizations of CSFV E rns and BVDV tE2 -based ELISA procedures. A & C Optimization of the concentration of coating antigen and the dilution of swine sera for CSFV E rns ( A ) or BVDV tE2 ( C ) -based ELISA using checkerboard titration test. B & D Optimization of the dilution of the secondary antibody for CSFV E rns ( B ) or BVDV tE2 ( D ) -based ELISA. P/N, positive control/negative control; ☆, the optimized condition for ELISA test

    Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Titration, Positive Control, Negative Control

    Validation of the specificity of CSFV E rns and BVDV tE2 -based indirect ELISAs. The specificities of CSFV E rns -based ( A , C & E ) and BVDV tE2 -based ELISAs ( B , D & F ) were validated using a panel of infected swine sera ( A & B ), including PCV2 (n = 9), PRRSV (n = 6), PEDV (n = 4), ASFV (n = 5), CSFV (n = 10), BVDV (n = 10) and negative control (n = 6); a panel of immunized rabbit sera (C & D), including CSFV (n = 4), BVDV (n = 4) and negative control (n = 4) or immunized mouse sera ( E & F ), including CSFV (n = 6), BVDV (n = 6) and negative control (n = 6)

    Journal: Virology Journal

    Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

    doi: 10.1186/s12985-022-01851-w

    Figure Lengend Snippet: Validation of the specificity of CSFV E rns and BVDV tE2 -based indirect ELISAs. The specificities of CSFV E rns -based ( A , C & E ) and BVDV tE2 -based ELISAs ( B , D & F ) were validated using a panel of infected swine sera ( A & B ), including PCV2 (n = 9), PRRSV (n = 6), PEDV (n = 4), ASFV (n = 5), CSFV (n = 10), BVDV (n = 10) and negative control (n = 6); a panel of immunized rabbit sera (C & D), including CSFV (n = 4), BVDV (n = 4) and negative control (n = 4) or immunized mouse sera ( E & F ), including CSFV (n = 6), BVDV (n = 6) and negative control (n = 6)

    Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

    Techniques: Biomarker Discovery, Infection, Negative Control

    The sensitivity of CSFV E rns and BVDV tE2 -based ELISAs. A Evaluation of the sensitivity of CSFV E rns -based ELISA using serially swine sera with different neutralizing antibody (NAb) titers. P1, P2 and P3, anti-CSFV positive sera; N1, CSFV-free serum. The number in brackets indicated the NAb titer. B Evaluation of the sensitivity of BVDV tE2 -based ELISA using serially swine sera with different NAb titers. P1, P2 and P3, anti-BVDV positive sera; N1, BVDV-free negative serum. The number in brackets indicated the NAb titer

    Journal: Virology Journal

    Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

    doi: 10.1186/s12985-022-01851-w

    Figure Lengend Snippet: The sensitivity of CSFV E rns and BVDV tE2 -based ELISAs. A Evaluation of the sensitivity of CSFV E rns -based ELISA using serially swine sera with different neutralizing antibody (NAb) titers. P1, P2 and P3, anti-CSFV positive sera; N1, CSFV-free serum. The number in brackets indicated the NAb titer. B Evaluation of the sensitivity of BVDV tE2 -based ELISA using serially swine sera with different NAb titers. P1, P2 and P3, anti-BVDV positive sera; N1, BVDV-free negative serum. The number in brackets indicated the NAb titer

    Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

    Techniques: Enzyme-linked Immunosorbent Assay

    Comparison of the BVDV tE2 -based indirect ELISA with virus neutralization test for detection of clinical serum samples of pigs

    Journal: Virology Journal

    Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

    doi: 10.1186/s12985-022-01851-w

    Figure Lengend Snippet: Comparison of the BVDV tE2 -based indirect ELISA with virus neutralization test for detection of clinical serum samples of pigs

    Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

    Techniques: Comparison, Indirect ELISA, Virus, Neutralization, Enzyme-linked Immunosorbent Assay